Actually it's from Star Wars Episode V - The Empire Strikes Back, and the quotee is of course Darth Vader...
EDIT: and watching through that great scene right now, I can even correct the quote... It's not "Luke, I'm your father"... it goes like this:
Quote:
DV: Obi-Wan never told you what happened to your father.
LS: He told me enough! He told me you killed him!
DV: No. I am your father.
LS: No. No. It's not true. That's impossible!
DV: Search your feelings. You know it to be true.
LS: Nooooooo! Noooooooo!
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Gosh, that's one of the greatest movie moments ever! Of course all while the Darth Vader theme, "Empiral March", plays in the background... I still get goosebumps!
EDIT: and checking up on imdb show they've got the quote wrong! Oh it's just a minor thing, missing a "No" and have "That's not true" instead of "It's not true"... But a movie trivia freak like me gotta submit a correction!!! :whistle: