Do we know if this could happen with other settings for machine different from the default, besides vgaonly? Also it may depend on other settings like scaler, or perhaps if you had aspect=true it would be 640x480 instead of 400? We can simply warn that fiddling with machine can have this effect.
According to the post in Vogons, this behavior is by design, necessary to support something. You should be able to convert the image file back to paletted, as long as you used a normal scaler, and not one that adds new colors by interpolation (if the scaler really influences these captures). Any image processing software has an algorithm to generate the optimal palette for an image, and if an image does not have more than 256 colors the optimal 256 colors palette generated should have the same exact colors as the original.
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